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METHODS

 Detection of Contaminants
 
  4.1Detection of Contaminants in Wort
  4.1.1Wort Forcing Test
  4.1.2General Aerobic Count
  4.1.3Specific Detection of Bacteria in Wort
  4.1.3.1Enterobacteriaceae
  4.1.3.2Lactic Acid Bacteria
  4.2Detection of Contaminants in Yeast and Fermenting Beer
  4.2.1Enrichment
  4.2.2General Aerobic Count on Samples of Yeast or Fermenting Beer
  4.2.3General Anaerobic Count on Samples of Yeast or Fermenting Beer
  4.2.4Specific Detection of Bacteria in Yeast
  4.2.4.1Enterobacteriaceae
  4.2.4.2Lactobacillus and Pediococcus
  4.2.4.3Acetic Acid Bacteria
  4.2.5Saccharomyces Wild Yeasts
  4.2.5.1Cu-differentiation
  4.2.5.2Heat Differentiation
  4.2.6Non-Saccharomyces Yeasts
  4.2.7Dekkera (formerly Brettanomyces)
  4.3Detection of Contaminants in Beer
  4.3.1Shelf Life Test
  4.3.1.1Shelf Life of Beer in Bottles
  4.3.1.2Shelf Life of Beer in Cans and Kegs
  4.3.1.3Shelf Life of Kegged Beer Transferred to Bottles
  4.3.1.4Enrichment
  4.3.2Non-Specific Detection of Microorganisms in Beer
  4.3.2.1General Aerobic Count on Beer
  4.3.2.2General Anaerobic Count on Beer
  4.3.3Specific Detection of Bacteria in Beer
  4.3.3.1Lactic Acid Bacteria
  4.3.3.2Pectinatus and Megasphaera
  4.3.3.3Selective enrichment of Pectinatus and Megasphaera
  4.4Detection of Contaminants in Water
  4.4.1General Aerobic Count in Water
  4.4.2Escherichia coli and Coliform Bacteria
  4.4.3Enterococci
  4.4.4Chromogenic/Fluorogenic Method for Escherichia coli and Coliform Bacteria
  4.4.5Clostridium perfringens (including spores) in water
  4.5Detection of Contaminants in Barley and Malt
  4.5.1Fusarium
  4.5.2Storage Fungi
  4.5.3General Method - Cultivation on Wet Filter Paper
  4.5.6Total aerobic count of yeast and bacteria in malt and barley
  4.5.7Detection and enumeration of presumptive coliforms in malt and barley
  4.5.8Detection and Enumeration of Salmonella in Malt, Barley and Brewers’ grains
  4.5.9Total aerobic count of yeast and bacteria in Brewers grains
  4.6Detection of Contaminants in other Brewing Materials
  4.6.1Detection of Contaminants in Additives
  4.6.2General Aerobic Count in Dilute Sugars
  4.6.3General Aerobic Count in Process Gases
  4.7Plant Hygiene
  4.7.1General Aerobic Count in Acid and Caustic Recovery Systems
  4.7.2Microbiological Evaluation of the Efficacy of Sterilants and Biocides